ABSTRACT
<p><b>OBJECTIVE</b>To investigate the anti-mutagenic action of the total flavonoids of Astragalus (TFA).</p><p><b>METHOD</b>Three groups of concentrations of TFA and one inducer group were used. The anti-mutagenic action of TFA was studied by experiments in vitro and in vivo including miconucleolus assay, semen teraogenesis test and gene mutagenesis of V79/HGPRT.</p><p><b>RESULT</b>TFA did not affect the weight of the mice during the experiment period. The high concentration of TFA could significantly reduce cyclohophamide-induced miconucleolus number and gene mutagenesis of V79/HGPRT compared with inducer group. However, TFA did not make statistic change in the semen teraogenesis induced by mitomycin C.</p><p><b>CONCLUSION</b>TFA has antimutagenesis effect.</p>
Subject(s)
Animals , Cricetinae , Male , Mice , Antimutagenic Agents , Pharmacology , Astragalus propinquus , Chemistry , Cells, Cultured , Chromosome Aberrations , Cricetulus , Flavonoids , Pharmacology , Micronucleus Tests , Mutagenesis , Plants, Medicinal , Chemistry , Testis , Cell BiologyABSTRACT
<p><b>OBJECTIVE</b>To evaluate bi-directional modulation effect of Chinese herbal medicine on immunol cells.</p><p><b>METHOD</b>Two different active portions were isolated from Kudzuvine Root(Radix puerariae), one being the ethanol extraction and another the water extraction. Different concentration of these two different portions was studied by using PMA stimulated lymphocyte or eosinophil initiated chemiluminescence system.</p><p><b>RESULT</b>Water extraction of Kudzuvine Root could enhance chemiluminescence concentration dependently whereas enthanol extraction of Kudzuvine Root inhibited the chemiluminescence significantly.</p><p><b>CONCLUSION</b>The bi-directional regulation effect of Chinese herbal medicine can be found in the same herb, because of its efficacy of different active compounds.</p>